Table 1.
Comparison of reported kinetic parameters of ATIC from multiple species
| Species | Vmax (AICAR) (U/mg) | kcat_app(AICAR) (s−1) | Km_app(AICAR) (μM) | Vmax(FAICAR) (U/mg) | kcat_app(FAICAR) (s−1) | Km_app (FAICAR) (μM) | Reference |
|---|---|---|---|---|---|---|---|
| Cryptococcus neoformans | 6.7 ± 0.1 | 7.5 ± 0.1 | 130 ± 10 | 8.6 ± 0.1 | 7.7 ± 0.1 | 30 ± 1 | This work |
| Saccharomyces cerevisiae | |||||||
| Ade16 | 1.0 ± 0.1 | ND | 26 | 1.7 ± 0.4 | ND | ND | (23) |
| Ade17 | 0.9 ± 0.1 | 22 | 2.0 ± 0.5 | ||||
| Homo sapiens | 1.7 ± 0.6 | 2.9 ± 0.4 | 16.8 ± 1.5 | 2.8 ± 0.01 | 6.0 ± 0.8 | 1.4 ± 0.1 | (22) |
| Gallus gallus | 2.7 ± 0.2 | ND | 15.2 ± 3.6 | ND | ND | ND | (39) |
| Staphylococcus lugdunensis | 0.5 ± 0.0 | ND | 32.4 ± 2.5 | 2.6 ± 0.5 | ND | 2.1 ± 0.3 | (37) |
Abbreviation: ND, no data.
Kinetic parameters for AICAR transformylase and IMP cyclohydrolase activities of C. neoformans ATIC, compared with published values from other species. Values are shown ± standard error.