Fig. 6.

AC anatomy and functions are maintained upon mesodermal-RNAi knockdown of identified genes. Hermaphrodite animals with the mesodermal-RNAi system and a LAM-1::mCherry reporter transgene were treated with RNAi under conditions that produce a phenotype and were selected as L3s and evaluated for AC morphology and capacity to mediate breakdown of the basement membrane between the AC and the dividing VPCs. The presence and timing of basement membrane breakdown (as indicated by clearance of LAM-1::mCherry) between the tissues was maintained under RNAi knockdown of all representative genes from each functional group. n = 10 for each RNAi knockdown. Images illustrate representative animals, and no defects were observed.