Fig. 7.

GV1001 has anti-tumor activity against A549 and H1299 cells. Quiescent cells were treated with GV1001 (0.05–5 μM) for 30 min, followed by 10% FBS stimulation for (A, C) 24 h, (B) 18 h or (D) 48 h. (A) The results from at least three independent experiments (mean ± SD) are presented as the fold-increase of untreated controls. (B) Results from at least three independent experiments (mean ± SD) are presented as the numbers of invasive cells. (C) RT-PCR analysis was performed as described in Materials and methods. Results are representative of at least three independent experiments. (D) ELISA assay was performed using conditioned media from cell culture treated as described above. Values represent the mean ± SD of at least three independent experiments. Statistical significance is indicated (*p < 0.05, **p < 0.01, compared with 10% FBS-treated cells).