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. 2022 Sep 27;3(4):101727. doi: 10.1016/j.xpro.2022.101727
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

Ketamine hydrochloride (100 mg/mL) Covetrus NDC: 11695-0703-1
Xylazine injection sterile solution (20 mg/mL) Akorn Family Health NDC: 59399-110-20
NADA# 139-236
Dulbecco’s phosphate-buffered salt solution 1× Corning Mfr# 21-031-CV
EDTA disodium salt Research Products International CAS# 6381-92-6
Sodium hydroxide (pellets) Fisher Chemical Cat# S318-500
Neutral buffered formalin solution 10% Sigma-Aldrich HT50-1-128
Paraformaldehyde 4% w/v aqueous solution Thermo Scientific Mfr# 473929M
RNAlater Stabilization Solution Thermo Scientific Cat# AM7020
TRIzol Reagent Thermo Scientific Cat# 15596026
Chloroform Thermo Scientific Cat# J67241-AP
Hematoxylin solution, Mayer’s MilliporeSigma SKU# MHS16-500ML PubChem Substance ID: 24897407
Eosin Y solution, alcoholic MilliporeSigma SKU# HT110116-500ML
Reagent alcohol MilliporeSigma SKU# 362808-1L Pubchem Substance ID: 329755001
Xylenes MilliporeSigma CAS# 1330-20-7 Pubchem Substance ID: 329752157

Critical commercial assays

Quick-DNA Miniprep Plus Kit Zymo Research Cat# D4068
DNA Clean & Concentrator-5 Zymo Research Cat# D4003
RNA Clean & Concentrator-5 (DNase included) Zymo Research Cat# R1013

Experimental models: Organisms/strains
C57BL/6J female mice, 8–12 weeks old The Jackson Laboratory Strain #: 000664
RRID: IMSR_JAX:000664

Other

1cc Insulin syringe U-100 29 Gauge Becton Dickinson 309311
Instant sealing sterilization pouches Fisher Scientific Cat# 01-812-51
Curved scissors Fine Science Tools Cat# 14085-08
Dissector scissors Fine Science Tools Cat# 14082-09
Short fine blade straight scissors Excelta Mfr# 288
Forceps Dumont #5 Fine Tips Fine Science Tools Cat# 11254-20
Straight broad strong tip general application forceps Fisher Scientific Cat# 11-100-107
General purpose pinning forceps Fisher Scientific Cat# 10-270
1 mm biopsy punch (25/pack) Acuderm Inc. Cat# P150
2 mm biopsy punch (25/pack) Acuderm Inc. Cat# P250
Cotton tipped applicators, 6″ wood handle Fisherbrand Cat# 14-960-3Q
Gauze sponges Fisher Scientific Cat# 13-761-52
Sterile alcohol prep pads Fisherbrand Cat# 22-363-750
Absorbant underpads, mats Fisherbrand Cat# 1420662
Animal warming pad N/A N/A
Warming lamp N/A N/A
Puralube vet ophthalmic (eye) ointment MWI Veterinary Mfr# 027505
P1000 1,000 μL pipette tips N/A N/A
Razor blade 0.0009″ Personna Mfr# 94-0491-0000
Richard-Allan paraffin wax – Type 9 Thermo cientific Cat# 8337
Glass 20 mL scintillation vials and Urea caps DKW Life Sciences Mfr# 986562
Accu-Edge low profile microtome blades Sakura Mfr# 4689
Tissue path IV tissue cassettes Fisher Scientific Cat# 22-272416
Fisherbrand Superfrost plus microscope slides Fisher Scientific Cat# 12-550-15
Tissue-Tek stainless steel base mold Sakura Cat# 4162
Round toothpicks N/A N/A
Pencil N/A N/A
pH meter N/A N/A
Roboz surgical germinator 500 bead sterilizer Roboz Surgical Mfr# DS401
Light microscope N/A N/A
Microtome N/A N/A
Floatation bath for tissueprep Fisher N/A
Paraffin embedding station N/A N/A
General purpose compact oven Thermo Scientific Mfr# PR305220G
Microscope slide drying rack N/A N/A
Autoclave machine N/A N/A
Paraffin wax melting pot N/A N/A