| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Ketamine hydrochloride (100 mg/mL) | Covetrus | NDC: 11695-0703-1 |
| Xylazine injection sterile solution (20 mg/mL) | Akorn Family Health | NDC: 59399-110-20 NADA# 139-236 |
| Dulbecco’s phosphate-buffered salt solution 1× | Corning | Mfr# 21-031-CV |
| EDTA disodium salt | Research Products International | CAS# 6381-92-6 |
| Sodium hydroxide (pellets) | Fisher Chemical | Cat# S318-500 |
| Neutral buffered formalin solution 10% | Sigma-Aldrich | HT50-1-128 |
| Paraformaldehyde 4% w/v aqueous solution | Thermo Scientific | Mfr# 473929M |
| RNAlater Stabilization Solution | Thermo Scientific | Cat# AM7020 |
| TRIzol Reagent | Thermo Scientific | Cat# 15596026 |
| Chloroform | Thermo Scientific | Cat# J67241-AP |
| Hematoxylin solution, Mayer’s | MilliporeSigma | SKU# MHS16-500ML PubChem Substance ID: 24897407 |
| Eosin Y solution, alcoholic | MilliporeSigma | SKU# HT110116-500ML |
| Reagent alcohol | MilliporeSigma | SKU# 362808-1L Pubchem Substance ID: 329755001 |
| Xylenes | MilliporeSigma | CAS# 1330-20-7 Pubchem Substance ID: 329752157 |
| Critical commercial assays | ||
| Quick-DNA Miniprep Plus Kit | Zymo Research | Cat# D4068 |
| DNA Clean & Concentrator-5 | Zymo Research | Cat# D4003 |
| RNA Clean & Concentrator-5 (DNase included) | Zymo Research | Cat# R1013 |
| Experimental models: Organisms/strains | ||
| C57BL/6J female mice, 8–12 weeks old | The Jackson Laboratory | Strain #: 000664 RRID: IMSR_JAX:000664 |
| Other | ||
| 1cc Insulin syringe U-100 29 Gauge | Becton Dickinson | 309311 |
| Instant sealing sterilization pouches | Fisher Scientific | Cat# 01-812-51 |
| Curved scissors | Fine Science Tools | Cat# 14085-08 |
| Dissector scissors | Fine Science Tools | Cat# 14082-09 |
| Short fine blade straight scissors | Excelta | Mfr# 288 |
| Forceps Dumont #5 Fine Tips | Fine Science Tools | Cat# 11254-20 |
| Straight broad strong tip general application forceps | Fisher Scientific | Cat# 11-100-107 |
| General purpose pinning forceps | Fisher Scientific | Cat# 10-270 |
| 1 mm biopsy punch (25/pack) | Acuderm Inc. | Cat# P150 |
| 2 mm biopsy punch (25/pack) | Acuderm Inc. | Cat# P250 |
| Cotton tipped applicators, 6″ wood handle | Fisherbrand | Cat# 14-960-3Q |
| Gauze sponges | Fisher Scientific | Cat# 13-761-52 |
| Sterile alcohol prep pads | Fisherbrand | Cat# 22-363-750 |
| Absorbant underpads, mats | Fisherbrand | Cat# 1420662 |
| Animal warming pad | N/A | N/A |
| Warming lamp | N/A | N/A |
| Puralube vet ophthalmic (eye) ointment | MWI Veterinary | Mfr# 027505 |
| P1000 1,000 μL pipette tips | N/A | N/A |
| Razor blade 0.0009″ | Personna | Mfr# 94-0491-0000 |
| Richard-Allan paraffin wax – Type 9 | Thermo cientific | Cat# 8337 |
| Glass 20 mL scintillation vials and Urea caps | DKW Life Sciences | Mfr# 986562 |
| Accu-Edge low profile microtome blades | Sakura | Mfr# 4689 |
| Tissue path IV tissue cassettes | Fisher Scientific | Cat# 22-272416 |
| Fisherbrand Superfrost plus microscope slides | Fisher Scientific | Cat# 12-550-15 |
| Tissue-Tek stainless steel base mold | Sakura | Cat# 4162 |
| Round toothpicks | N/A | N/A |
| Pencil | N/A | N/A |
| pH meter | N/A | N/A |
| Roboz surgical germinator 500 bead sterilizer | Roboz Surgical | Mfr# DS401 |
| Light microscope | N/A | N/A |
| Microtome | N/A | N/A |
| Floatation bath for tissueprep | Fisher | N/A |
| Paraffin embedding station | N/A | N/A |
| General purpose compact oven | Thermo Scientific | Mfr# PR305220G |
| Microscope slide drying rack | N/A | N/A |
| Autoclave machine | N/A | N/A |
| Paraffin wax melting pot | N/A | N/A |