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. 2022 Sep 22;16:100438. doi: 10.1016/j.mtbio.2022.100438

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© 2022 Published by Elsevier Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — The programmed delivery system promoted osteogenic mineralization in an inflammatory environment in vitro. (A) Images of immunofluorescence staining showing the expression of Runx2 of BMSCs (n = 3). (B) Corresponding quantitative results (n = 3). (C) Osteogenic gene expression in BMSCs, analyzed by qRT-PCR (n = 3). (D) Representative images and quantitative analysis of alkaline phosphatase staining (n = 3). (E) Western blot of Col I, Runx2, OCN, and OPN expression in BMSCs and statistical analysis (n = 3). ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001, compared with the blank group.