Fig. 6.

The programmed delivery system obviously reduced the inflammation of periodontitis and enhanced bone regeneration in vivo. (A) Representative images of H&E staining at 1 and 4 weeks. (B) Representative images of Masson staining at 1 week. (C) Representative images of immunohistochemistry staining at 1 week; the red arrow indicates iNOS-positive cells. (D) Representative images of TRAP staining at 1 week; the yellow arrow indicates osteoclasts. (E) Quantitative analysis of iNOS-positive cells and TRAP-positive cells (n ​= ​5). (F) The Relative levels of inflammatory cytokines in the serum at 1 and 2 weeks (n ​= ​5). (G) Western blot of periodontal bone tissue near the second molar at 1 and 2 weeks. ∗P ​< ​0.05, ∗∗P ​< ​0.01 and ∗∗∗P ​< ​0.001, compared with the blank group; FM ​= ​first molar; SM ​= ​second molar; AB ​= ​alveolar bone. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)