TABLE 1.

Lipid composition of HCT 116 2D monolayers and 3D spheroid regions

Subclass	2D (%mol ± SD)	Outer (%mol ± SD)	Middle (%mol ± SD)	Core (%mol ± SD)
FA	53.24 ± 2.80	56.99 ± 0.99	55.09 ± 2.83	57.88 ± 2.44
PC	30.45 ± 1.88	28.71 ± 2.07	28.91 ± 2.39	28.71 ± 1.25
Ether PC	9.79 ± 0.61	5.38 ± 0.49	8.04 ± 0.54	6.16 ± 0.61
PI	2.99 ± 0.28	2.96 ± 0.22	3.15 ± 0.26	3.14 ± 0.32
DG	1.06 ± 0.07	1.63 ± 0.60	1.23 ± 0.31	1.09 ± 0.10
Lysolipids	1.01 ± 0.31	2.24 ± 1.49	0.95 ± 0.32	0.64 ± 0.39
Ether PE	0.54 ± 0.04	0.76 ± 0.03	0.79 ± 0.05	0.78 ± 0.08
PE	0.37 ± 0.06	0.48 ± 0.05	0.43 ± 0.05	0.46 ± 0.06
TG	0.08 ± 0.01	0.28 ± 0.07	0.64 ± 0.03	0.45 ± 0.05
DMPE	0.16 ± 0.02	0.16 ± 0.01	0.17 ± 0.02	0.17 ± 0.02
SM	0.11 ± 0.01	0.13 ± 0.01	0.16 ± 0.01	0.15 ± 0.02
PS	0.13 ± 0.01	0.12 ± 0.01	0.13 ± 0.01	0.14 ± 0.01
Cer	0.03 ± 0.01	0.07 ± 0.01	0.17 ± 0.02	0.09 ± 0.02
SHexCer	0.03 ± 0.01	0.05 ± 0.004	0.07 ± 0.01	0.06 ± 0.01
CAR	4.0E‐03 ± 0.001	1.8E‐02 ± 0.01	2.6E‐02 ± 0.004	5.2E‐02 ± 0.03
HexCer	5.1E‐03 ± 0.001	1.4E‐02 ± 0.002	2.4E‐02 ± 0.004	2.3E‐02 ± 0.004
PG	4.7E‐03 ± 0.002	8.9E‐03 ± 0.001	1.2E‐02 ± 0.004	9.2E‐03 ± 0.002
NAE	3.3E‐04 ± 1.9E‐05	5.1E‐04 ± 5.2E‐05	9.5E‐04 ± 1.3E‐04	9.6E‐04 ± 9.4E‐05