Figure S5.

LLPS capacity of truncation mutants of CGB. (A) Images obtained by plating 10 µM of either CGB-GFP or CGB_Nterm-GFP or CGB_Cterm-mCherry without any crowding agent. Upon equilibration at pH 6.1 Note that droplet formation is seen only in CGB-GFP solution under these conditions. (B) Images obtained by plating of 2 µM of CGB-GFP or CGB_Nterm-GFP or CGB_Cterm-GFP in presence of 3% PEG 8000 to monitor droplet formation in these conditions. Only a few small droplets are observed in CGB_Cterm-GFP as compared to CGB-GFP or CGB_Nterm-GFP. (C) Western blots to compare the expression levels of CGB_Nterm-GFP and CGB_Cterm-GFP from HEK293 cells stably expressing these constructs upon induction using doxycycline and probed using GFP antibody (top). β-actin (bottom) was used as a loading control. Source data are available for this figure: SourceData FS5.