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. 2022 Sep 29;221(12):e202206132. doi: 10.1083/jcb.202206132

Table 1.

Sequences of primers used in this study

Primer name Sequence
CGA_sfGFP_6XHis F1 FP 5′-GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​CTA​GCA​TGC​GCT​CCG​CCG​CTG​TCC-3′
CGA_sfGFP_6XHis F1 RP 3′-CCG​GTG​GCG​ACC​GGT​GGA​TCC​AAG​CCC​CGC​CGT​AGT​GCC​TGC-5′
CGA_sfGFP_6XHis 2 FP 5′-GCA​GGC​ACT​ACG​GCG​GGG​CTT​GGA​TCC​ACC​GGT​CGC​CAC​CGG-3′
CGA_sfGFP_6XHis F2 RP 3′-CCA​GCA​CAC​TGG​ATC​AGT​TAT​CTA​TGC​GGC​CGC​TCA​GTG​ATG​ATG​ATG​GTG​ATG​GCT​GCC​CTT​GTA​CAG​CTC​GTC​CAT​GCC-5′
CGB_sfGFP_6XHis F1 FP 5′-GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​CTA​GCA​TGC​AGC​CAA​CGC​TGC​TTC​TCA​GCC​TC-3′
CGB_sfGFP_6XHis F1 RP 3′-CAC​CGG​TGG​CGA​CCG​GTG​GAT​CCA​AGC​CCC​TTT​GGC​TGA​ATT​TCT​CAG​CTA​TCT​TCT​GTA​GTT​CC-5′
CGB_sfGFP_6XHis F2 FP 5′-GGA​ACT​ACA​GAA​GAT​AGC​TGA​GAA​ATT​CAG​CCA​AAG​GGG​CTT​GGA​TCC​ACC​GGT​CGC​CAC​CGG-3′
CGB_sfGFP_6XHis F2 RP 3′-CCA​GCA​CAC​TGG​ATC​AGT​TAT​CTA​TGC​GGC​CGC​TCA​TTA​GCT​GCC​CTT​GTA​CAG​CTC​GTC​CAT​GCC-5′
CGA_mEGFP_6XHis F1 FP 5′-GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​CTA​GCA​TGC​GCT​CCG​CCG​CTG​TCC-3′
CGA_mEGFP_6XHis F1 RP 3′-AAC​AGC​TCC​TCG​CCC​TTG​CTC​ACC​ATA​GCT​CCT​GCA​CCG​GTG​GCG​ACC-5′
CGA_mEGFP_6XHis F2 FP 5′-GGT​CGC​CAC​CGG​TGC​AGG​AGC​TAT​GGT​GAG​CAA​GGG​CGA​GGA​GCT​GTT-3′
CGA_mEGFP_6XHis F2 RP 3′-CCA​GCA​CAC​TGG​ATC​AGT​TAT​CTA​TGC​GGC​CGC​TCA​GTG​ATG​ATG​ATG​GTG​ATG​CTT​GTA​CAG​CTC​GTC​CAT​GCC​GAG​AGT​GAT​CCC-5′
CGB_5(ED)/A_sfGFP_6XHis F1 FP 5′-GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​CTA​GCA​TG-3′
CGB_5(ED)/A_sfGFP_6XHis F1 RP 3′-CAC​CGG​TGG​CGA​CCG​GTG​GAT​C-5′
CGB_5(ED)/A_sfGFP_6XHis F2 FP 5′-GCT​TCA​GAA​AAT​AGC​GGA​GAA​GTT​CTC​ACA​ACG​AGG​CTT​GGA​TCC​ACC​GGT​CGC​CAC​CGG-3′
CGB_5(ED)/A_sfGFP_6XHis F2 RP 3′-CCA​GCA​CAC​TGG​ATC​AGT​TAT​CTA​TGC​GGC​CGC​TCA​GTG​ATG​ATG​ATG​GTG​ATG​GCT​GCC​CTT​GTA​CAG​CTC​GTC​CAT​GCC-5′
CGB_N term_sfGFP_6XHis F1 FP 5′-GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​CTA​GCA​TGC​AGC​CAA​CGC​TGC​TTC​TCA​GCC​TC-3′
CGB_N term_sfGFP_6XHis F1 RP 3′-CAC​CGG​TGG​CGA​CCG​GTG​GAT​CCA​AAG​CCC​TGT​AGT​GGG​TTG​AAT​GGT​GGT​CCC-5′
CGB_N term_sfGFP_6XHis F2 FP 5′-GGG​ACC​ACC​ATT​CAA​CCC​ACT​ACA​GGG​CTT​TGG​ATC​CAC​CGG​TCG​CCA​CCG​G-3′
CGB_N term_sfGFP_6XHis F2 RP 3′-CCA​GCA​CAC​TGG​ATC​AGT​TAT​CTA​TGC​GGC​CGC​TCA​GTG​ATG​ATG​ATG​GTG​ATG​GCT​GCC​CTT​GTA​CAG​CTC​GTC​CAT​GCC-5′
CGB_C term_sfGFP_6XHis F1 FP 5′-GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​CTA​GCA​TG-3′
CGB_C term_sfGFP_6XHis F1 RP 3′-CAC​CGG​TGG​CGA​CCG​GTG​GAT​C-5′
CGB_C term_sfGFP_6XHis F2 FP 5′-GGA​ACT​ACA​GAA​GAT​AGC​TGA​GAA​ATT​CAG​CCA​AAG​GGG​CTT​GGA​TCC​ACC​GGT​CGC​CAC​CGG​TG-3′
CGB_C term_sfGFP_6XHis F2 RP 3′-CCA​GCA​CAC​TGG​ATC​AGT​TAT​CTA​TGC​GGC​CGC​TCA​GTG​ATG​ATG​ATG​GTG​ATG​GCT​GCC​CTT​GTA​CAG​CTC​GTC​CAT​GCC-5′
CGB_C term_mCherry_6XHis F1 FP 5′-GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​CTA​GCA​TG-3′
CGB_C term_mCherry_6XHis F1 RP 3′-CAC​CGG​TGG​CGA​CCG​GTG​GAT​C-5′
CGB_C term_mCherry_6XHis F2 FP 5′-TTG​GAT​CCA​CCG​GTC​GCC​ACC​GGT​GCA​GGA​GCT​ATG​GTG​AGC​AAG​GGC​GAG​GAG​GAT​AAC​ATG​G-3′
CGB_C term_mCherry_6XHis F2 RP 3′-CCA​GCA​CAC​TGG​ATC​AGT​TAT​CTA​TGC​GGC​CGC​TCA​GTG​ATG​ATG​ATG​GTG​ATG​CTT​GTA​CAG​CTC​GTC​CAT​GCC​GCC​G-5′
pLenti_LyzC_EGFP_attB_FP 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTA​TGA​AGG​CTC​TCA​TTG​TTC​TGG​GGC​TTG​TCC​TCC-3′
pLenti_LyzC_EGFP_attB_RP 3′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TAC​TTG​TAC​AGC​TCG​TCC​ATG​CCG​AGA​GTG​ATC​CCG-5′
pLenti_CatD_sfGFP_F1_FP 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTA​TGC​AGC​CCT​CCA​GCC​TTC​TGC​CGC​TCG​CCC​TCT​GCC​TGC​TGG​CTG​CAC​CCG​CCT​CCG​CGC​TCG​TCA​GGA​TCC​CGC​TGC​ACA​AGT​TCA-3′
pLenti_CatD_sfGFP_F1_RP 3′-ACC​GGT​GGC​GAC​CGG​TGG​ATC​CAA​GAG​GCG​GGC​AGC​CTC​GGC​GA-5′
pLenti_CatD_sfGFP_F2_FP 5′-TCG​CCG​AGG​CTG​CCC​GCC​TCT​TGG​ATC​CAC​CGG​TCG​CCA​CCG​GT-3′
pLenti_CatD_sfGFP_F2_RP 3′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TAG​CTG​CCC​TTG​TAC​AGC​TCG​TCC-5′
SP_SBP_Halo_CGB_FP 5′-CGC​GCG​CTG​GCT​GTC​CAC​GCT​CGA​GAT​TTC​CGG​CGG​CGG​CAG​CAT​GCC​AGT​GGA​TAA​CAG​GAA​CCA​C-3′
SP_SBP_Halo_CGB_RP 3′-GCA​GCC​TGC​ACC​TGA​GGA​GTG​AAT​TCA​TTA​GCC​CCT​TTG​GCT​GAA​TTT​CTC-5′