Figure 1.

Schematic overview of the seven proposed QC guideline fields, together with the corresponding MetroloJ_QC tool icon. (a) PSF is a simple way to characterize the resolution power in the x, y, and z dimensions. (b) Field illumination determines the intensity quantification over the whole field of view of the system, tile-scan reconstructions, and inhomogeneous sample bleaching. (c) Coregistration characterizes the chromatic mismatch between different color channel images of the same fluorescent emitter in the x, y, and z directions. (d) Illumination power stability at different time scales determines the reproducibility and quantification of the experiment. (e) The imaging quality depends on the stability of the sample in x, y, and z characterized by drift monitoring and stage positioning repeatability. (f) The noise and offset characterization of the camera sensor influences quantification, especially for weak signals.