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. 2022 Sep 29;221(11):e202107093. doi: 10.1083/jcb.202107093

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© 2022 Faklaris et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 7. — Stage drift data summary. (A) Max Z projection of the time projection of a 15 h time-lapse of a fluorescent bead, and trajectory of the bead, with the initial segmented bead position shown in magenta. (B) Global 3D displacement over time. The arrow indicates the calculated stabilization time τ_stab. (C) Relative displacement along the xyz axes. (D) Calculated velocity before (V_b) and after (V_a) stabilization of different microscope stages grouped in three categories upon τ_stab. The dark green zone highlights the acceptable values. (E) xy time lapse over 600 s of living cells labeled with CD63PhLuorin (membrane labeling). (F) Overlay of the initial t = 0 (cyan) and final t = 600 s (magenta) frame of the series of E to highlight the relative drift along the x axis.