Figure S4.

Field illumination using either a green chroma plastic slide or a glass coverslide/coverslip configuration with a rhodamine layer for a spinning disk Dragonfly microscope (Andor–Oxford Instruments). (A) Field illumination images at 488 nm excitation for a 10× and 63× with a plastic and rhodamine slide, detected with an EMCCD iXon888 camera. Artifacts shown on these images come from a synchronization artifact between Nipkow disk rotation and camera acquisition. The image projected on the sensor chip results from the integration of multiple individual scans by the Nipkow disk. A short exposure time can result in these artifacts, as explained in (Chong et al., 2004). (B) Centering and uniformity metrics for A. The acquisition was set at the plastic/glass or rhodamine/coverslip interface, or 10/30 μm deep within the plastic/rhodamine layer. (C) Effect of focal plane depth on emission intensity using either a plastic slide or thin/thick layers of fluorescent rhodamine dye (PLAN APO 63×/1.4 objective, ex. 488 nm).