Figure S7.

Influence of the bead size on the coregistration accuracy calculated by MetroloJ_QC. (A–C) Co-registration ratio distributions for 4, 1, 0.5, and 0.2 μm multicolor beads. For all cases, more than five beads per condition and two FOV were examined, for a 100×/1.4 objective, on an upright WF microscope (AxioImager Z2, Carl Zeiss Microscopy GmbH). We observe that 0.2 μm beads present a high dispersion, with a coefficient of variation higher than 65% for all channel combinations. The 1 μm beads show the smallest dispersion for all cases and are thus the most adapted beads to use for this kind of measurements.