TABLE 1.
B. subtilis strains used in this study
| Strain | Relevant genotypea | Reference construction |
|---|---|---|
| CRK6000 | purA16 metB5 hisA3 guaB | 33 |
| NIS2000 | CRK6000 purA::Pspac-lacI-cat | Transformation of CRK6000 with a PCR product (see Materials and Methods) |
| NIS2010 | CRK6000 purA::PrpmH-lacO-lacI-cat | Same as above |
| NIS2020 | CRK6000 purA::PrpmH-lacO-dnaA-lacI-cat | Same as above |
| NIS6500 | CRK6000 amyE::pDLAH(PdinC-bgaB ermC) | 35 |
| NIS2065 | CRK6000 purA::PrpmH-lacO-dnaA-lacI-cat amyE::pDLAH(PdinC-bgaB ermC) | Transformation of NIS2020 with NIS6500 DNA |
| NIS6301 | CRK6000 spoIIIJ::pRK1(oriN cat) ΔoriC | 35 |
| NIS6311 | CRK6000 spoIIIJ::pRK1(oriN cat) dnaA(ochre) | 12, 17 |
| NIS2021 | CRK6000 purA::PrpmH-lacO-dnaA-dnaN-tet-pBR-lacI-cat | Transformation of NIS2020 with pSM5100 |
| NIS2022 | CRK6000 dnaA (ochre) purA::PrpmH-lacO-dnaA-dnaN-tet-pBR-lacI-neo | See Materials and Methods |
a
P, promoter region; oriN, minimal replication region of low-copy plasmid pLS32 (12).