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. 2022 Sep 28;5(12):e202201538. doi: 10.26508/lsa.202201538

Figure 4. Herbicide resistance analysis of transgenic rice plants expressing point mutations in the acetolactate synthase (ALS).

Figure 4.

(A) Nine mutant variants are generated for OsALS. Point mutations are introduced into the OsALS sequence using site-directed mutagenesis. Agrobacterium-mediated transformation was conducted of each of the p35S:ALS mutant variant. (B) Dose–response effects of bispyribac sodium (BS) treatment on germination of 35S:ALS-W548C, 35S:ALS-F575C, 35S:ALS-K591E, 35S:ALS-G629S, and 35S:ALS-G629R. Seeds of 35S:ALS with OsALS wild-type sequence was used as a control. Rice seeds were sterilized and germinated on ½ MS basal salt media plates for 2-wk with different concentrations of BS. Germination of 35S:ALS seeds was severely affected at 0.75 μM BS and completely inhibited at 1 μM BS. Germination of ALS mutant variants is less affected by 1 μM BS treatment. 35S:ALS-W548C germination is slightly affected even at 2 μM BS. Red arrows indicate the BS-resistant shoots. Three independent transgenic lines were tested for each of the ALS mutant variant.