FIG. 4.

TH uptake in MCT10 knockdown BeWo cells. (A) MCT10 mRNA expression in BeWo cells transfected with 20 nM MCT10 siRNA for 48 hours (data are represented as mean ± SEM of three experiments). (B, C) Time-dependent uptake of 1 nM T3 (B) and T4 (C) in BeWo cells transfected with 20 nM MCT10 siRNA or negative control siRNA for 48 hours (data are represented as mean ± SEM of three experiments performed in duplicate). Paired two-tailed t-test was used and statistical significance was compared to negative control. *0.01 < p ≤ 0.05; **0.001 < p ≤ 0.01. siRNA, small interfering RNA; T3, triiodothyronine; T4, thyroxine.