Table 3.
Antibody information.
| Antibody | Usage | Host-reactivity | Dilutions | Clonality | Source |
|---|---|---|---|---|---|
| PDGF-BB | Western blot | Rabbit anti-mouse | 1 : 500 | Polyclonal | Abcam, Cambridge, UK |
| PDGF-BB | Western blot | Chicken anti-human | 1 : 500 | Polyclonal | Abcam, Cambridge, UK |
| PDGFRβ | Western blot | Rabbit anti-mouse/human | 1 : 1000 | Monoclonal | Cell Signaling Technology, Danvers, MA, USA |
| Src | Western blot | Rabbit anti-mouse/human | 1 : 1000 | Monoclonal | Cell Signaling Technology, Danvers, MA, USA |
| p-Src | Western blot | Rabbit anti-mouse/human | 1 : 1000 | Monoclonal | Cell Signaling Technology, Danvers, MA, USA |
| Akt | Western blot | Rabbit anti-mouse/human | 1 : 1000 | Monoclonal | Cell Signaling Technology, Danvers, MA, USA |
| p-Akt | Western blot | Rabbit anti-mouse/human | 1 : 2000 | Monoclonal | Cell Signaling Technology, Danvers, MA, USA |
| CD31 | Immunofluorescence | Goat anti-mouse | 1 : 100 | Polyclonal | R&D Systems, Minneapolis, MN, USA |
| PDGFRβ | Immunofluorescence | Goat anti-mouse | 1 : 100 | Polyclonal | R&D Systems, Minneapolis, MN, USA |
| Anti-GFP | Immunofluorescence | Rabbit anti-GFP | 1 : 200 | Polyclonal | Abcam, Cambridge, UK |
| Alexa Fluor® 488 | Immunofluorescence | Goat anti-rabbit | 1 : 200 | Polyclonal | Abcam, Cambridge, UK |
| NL557 | Immunofluorescence | Donkey anti-goat | 1 : 500 | Polyclonal | R&D Systems, Minneapolis, MN, USA |