Figure 3.

SKQ1 treatment attenuates CDDP-induced mitochondrial dysfunction and lipid peroxidation-mediated ferroptosis in vivo. (a) Representative TEM images of kidneys from mice treated with cisplatin with or without SKQ1 administration (the red arrow indicates injured mitochondria). IHC photomicrographs of (b) 4-HNE and (c) GPX4 in kidney tissues treated under different conditions. The protein levels for (d) ATPB, SOD2, (e) MPO, and GPX4 isolated from renal sections in all mouse groups were quantified using WB. (f) The levels of LDH in the serum of all experimental groups were determined with a commercial kit. We also analyzed the (g) relative GSH/GSSG ratio and the (h) relative levels of MDA in the renal tissue lysates. Every quantified result was expressed as the mean ± SD for each group. ^∗∗∗∗P < 0.0001, ^∗∗∗P < 0.001, ^∗∗P < 0.01, and ^∗P < 0.05 (by one-way ANOVA).