Figure 7.

SKQ1 treatment attenuates renal ischemia-reperfusion injury. Relative levels of (a) BUN and (b) SCr in vehicle, vehicle+IRI, SKQ1, and SKQ1+IRI (n = 6) groups. (c) Serum LDH expression in all groups of IRI models. (d) Microstructure after PAS staining and representative renal tubular injury score for all mouse groups were analyzed. (e) TUNEL images (green, TUNEL; blue, DAPI) and relative fluorescence intensity from each kidney were determined. Representative immunoblots of (f) KIM-1, NGAL, (g) MPO, and GPX4 isolated from the same groups and relative results of densitometric analysis. (h) TEM images and quantification of damaged mitochondria (indicated by red arrow) in vehicle+IRI and SKQ1+IRI (n = 3) groups. (i) Relative expression of MDA in each group of IRI mice with or without SKQ1 administration (n = 6). ^∗∗∗∗P < 0.0001, ^∗∗∗P < 0.001, and ^∗∗P < 0.01 (by one-way ANOVA).