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. 2022 Oct 1;12:16473. doi: 10.1038/s41598-022-20141-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The extended IgG3 hinge results in increased neutralization potency in CAP88-CH06 antibodies. (a) CAP88-CH06 IgG3 (green) and IgA1 (purple) and IgG1 (blue) mAbs when tested for neutralization against 12 CAP88 autologous viruses in a pseudotyped virus neutralization assay. (b) Alignment of the CAP88-CH06 mAb hinge regions performed using Aliview v1.20. Red denotes amino acids that differ between hinge sequences mAbs and dashes represent gaps. (c) Antibody hinge chimeras were tested for neutralization against 12 CAP88 autologous viruses. All experiments were performed in duplicate with at least two replicates. Neutralization potency is shown as IC₅₀ values in μg/ml with neutralization resistance being defined as > 10 μg/ml. GMT geometric mean titre. Fold changes are shown in red (if greater than threefold different) or black (if less than threefold different). Statistical significance was calculated using the Friedman test with Dunns correction for multiple comparisons. Significance is shown as: *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Antibody schematics were created in Biorender (http://www.biorender.com).