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. 2022 Oct 1;12:16473. doi: 10.1038/s41598-022-20141-7

Figure 2.

Figure 2

The IgG3 hinge results in a minimal increase in ADCP and does not substantially affect ADCC in CAP88-CH06 antibodies. (a) Antibody-dependent cellular phagocytosis (ADCP) activity of the CAP88-CH06 IgG3 WT, IgG3_IgG1Hinge, IgG1 WT and IgG1_IgG3Hinge antibodies was measured using THP-1 phagocytosis assay and the phagocytosis score shown as an area under the curve (AUC) measure. (b) Antibody dependent cellular cytotoxicity (ADCC) activity of the CAP88-CH06 IgG3 WT, IgG3_IgG1Hinge, IgG1 WT and IgG1_IgG3Hinge antibodies was measured using an infectious ADCC assay. The % killing activity is shown as area under the curve (AUC) measure. In all experiments, HIVIG, a polyclonal plasma cocktail from HIV-infected individuals, was used as a positive control and Palivizumab, a RSV-specific mAb, was used as a negative control. Experiments were conducted in duplicate and error bars represent the mean with standard deviation of two experiments. The Kruskal–Wallis test with Dunn’s correction was performed and no comparisons were significantly different, except for the negative control, Palivizumab. Fold changes are shown in black.