Figure 3.
The IgA1 CH1 region results in increased neutralization potency in CAP88-CH06 antibodies. (a) Alignment of CAP88-CH06 mAb CH1 regions performed with Aliview v1.20. Red denotes amino acid differences between the CH1 sequences of the three mAbs. (b) Antibody CH1 chimeras were tested for neutralization against 12 CAP88 autologous viruses. All experiments were performed in duplicate with at least two replicates. Neutralization potency is shown as IC50 values in μg/ml with neutralization resistance being > 10 μg/ml. GMT geometric mean titre. Fold changes are shown in red (greater than threefold different) or black (less than threefold different). Statistical significance was calculated using the Friedman test with Dunns correction for multiple comparisons. Significance is shown as: *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Antibody schematics were created in Biorender (http://www.biorender.com).