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. 2022 Oct 1;5:1046. doi: 10.1038/s42003-022-04015-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic representing preparations of nine hydrated and nine preconditioned and desiccated tardigrade samples for metabolomics analysis. b Volcano plot displays differential enrichment of metabolites with the change in hydration state of tardigrades. c Summary table of the metabolome profiling comparing hydrated and desiccated tardigrade samples. Welch’s two-sample t-test used for statistical comparisons. d PCA showed major separation by hydration status on Component 1, suggesting that desiccation causes a substantial metabolic shift in tardigrades. e Random forest comparison between hydrated and desiccated groups in tardigrades identifies trehalose and trehalose precursor UDP-glucose as significant metabolites that contribute strongly to the group binning. The plot shows the mean decrease in binning accuracy that would result from the removal of a particular metabolite from our analysis. f RFA confusion matrix showing that predictive accuracy within our dataset is 100%. g Plot showing scaled intensity (relative metabolite abundance) for trehalose in hydrated and desiccated samples. n = 9, Welch’s two-sample t-test was used for statistical comparisons where *** indicates p-value < 0.001, error bars = standard deviation.