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. 2022 Oct 1;5:1046. doi: 10.1038/s42003-022-04015-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Yeast strain nth1Δ + TDH3pr-Agt1 was constructed to have a knockout of Nth1 which encodes trehalase for trehalose breakdown, and the addition of an intracellular trehalose transporter, AGT1. Yeast grown in log phase do not express Tps1 or Tps2. b–f nth1Δ + TDH3pr-Agt1 yeast strains expressing different CAHS genes with and without supplemented trehalose were assayed for survivability against desiccation stress. One way ANOVA was used for statistical comparisons where *** indicates p-value < 0.001, error bars = standard deviation.