Figure 3.

Vasoconstrictor responses of MCA segments following transfection with EGFP or EGFP–mβENaC. Vasoconstrictor responses to depolarizing agent (KCl, a) and α-adrenoceptor agonist agent (phenylephrine, PE, b) (n = 5) were not affected by expression of EGFP–mβENaC (●) compared to EGFP controls (○). Data represent internal diameters and were analyzed using a two-way repeated measures ANOVA, which showed an effect of KCl (P < 0.0001) and PE (P < 0.0001) concentration, but no effect of expression construct (P = 0.555, 0.729) or interaction (P = 0.335, 0.502). Data are presented as mean ± SEM, n = 5 in EGFP and EGFP–mβENaC groups. EGFP, enhanced green fluorescent protein; mβENaC, mouse beta-epithelial Na⁺ channel; MCA, middle cerebral artery; ANOVA, analysis of variance; SEM, standard error of the mean.