Figure 5.

Mechanical properties of MCA segments following transfection with EGFP and EGFP–mβENaC. Data were analyzed using a two-way repeated measures ANOVA, which showed an effect of intraluminal pressure (P < 0.0001), but no effect of expression construct (P = 0.0892) or interaction between pressure and construct (P = 0.2133). Data are presented as mean ± SEM, n = 5 in EGFP (○) and EGFP–mβENaC (●) groups. Sidak’s multiple comparison post hoc test showed that circumferential wall strain (a) and stress (b) under Ca²⁺-free conditions are identical between EGFP and EGFP–mβENaC MCAs. Data are presented as mean ± SEM, n = 5 in each group. EGFP, enhanced green fluorescent protein; mβENaC, mouse beta-epithelial Na⁺ channel; MCA, middle cerebral artery; ANOVA, analysis of variance; SEM, standard error of the mean.