Figure 5.
Triazoloquinazoline selectively prevents MERS-CoV proliferation by a similar mode of action as cyanofuran
(A) Representation of the structures of triazoloquinazoline (3) and its analogs.
(B) Summary of the potency of each analog. IC50 values of 3 and its analogs were determined by the dose-response curve obtained from the fluorescence-based protease assay, while the EC50 and CC50 were determined by an infection assay using the Vero cell MERS-CoV infection model.
(C) Representative immunofluorescence images treated with 3 and its analog 3a-1.
(D) RNA quantification of the viral upE gene in MERS-CoV-infected cells treated with compound 3. Each data point represents the mean of quadruple assays with ±SEM. ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.0005 compared to untreated control by Student’s t test.
(E) Molecular docking of triazoloquinazoline into MERS-PLpro (PDB: 4RF1) using Schrodinger XP Glide.