Table 4.

Liver average gene transcription levels (relative quantities; RQ) of ECS-related, lipid metabolism and inflammatory genes of PP dairy cows supplemented with n-3 fatty acids

Genes	Treatmenta		SEM	P-value
	Control	FLX
Endocannabinoid-related
CNR1b	0.05	0.03	0.007	0.11
CNR2c	0.71	0.46	0.083	0.07
MGLLd	2.65	2.24	0.528	0.60
NAPEPLDe	0.06	0.07	0.006	0.49
FAAHf	1.71	1.78	0.240	0.85
Lipid metabolism and beta oxidation
PPARAg	6.76	9.17	1.620	0.32
SREBP1h	1.22	1.26	0.135	0.87
MLXIPLi	1.45	1.64	0.310	0.67
ACOX1j	29.39	35.70	5.074	0.41
ACSL1k	18.28	13.74	3.413	0.37
FABP1l	83.21	116.75	16.969	0.20
CPT1Am	1.12	0.42	0.371	0.22
CPT2n	8.89	7.87	1.432	0.63
Inflammation
TNFAo	0.02	0.03	0.005	0.24
IL1Bp	0.06	0.07	0.014	0.81
IL6q	0.27	0.18	0.024	0.03
NFKB1r	0.18	0.22	0.031	0.46
SAA2s	65.99	28.32	26.738	0.35
HPt	38.42	10.17	12.899	0.16
IL10u	0.01	0.01	0.003	0.53

^aDairy cows were divided into two nutritional regiment groups from −21 to 60 days PP; 1) Control group (CTL)—a standard Israeli diet, 2) FLX—a standard diet supplemented with flaxseed oil containing n-3. ^bCannabinoid-1 Receptor; ^cCannabinoid-2 receptor; ^dMonoglyceride lipase; ^eN-Acyl phosphatidylethanolamine phospholipase D; ^fFatty acid amide hydrolase; ^gPeroxisome proliferator activated receptor alpha; ^hSterol regulatory element binding transcription factor 1; ⁱCarbohydrate response element binding protein; ^jAcyl-CoA oxidase; ^kAcyl-CoA synthetase long chain family member 1; ^lLiver fatty acid-binding protein; ^mCarnitine palmitoyltransferase 1; ⁿCarnitine palmitoyltransferase 2; ^oTumor necrosis factor α; ^pInterleukin 1 beta; ^qInterleukin 6; ^rNuclear factor kappa-light-chain-enhancer of activated B cells; ^sSerum amyloid A2; ^tHaptoglobin; ^uInterleukin 10