FIG. 4.

Primer extension analysis of the hpyIM transcript in the iceA1 H. pylori strain 60190. (A) The ³²P-labeled Meth3 primer was used in this analysis. The corresponding sequencing ladder is shown, and the arrow indicates the nucleotide position of the +1 site (residue A) that is 157 nt upstream of the hpyIM ORF (see Fig. 2). (B) The ³²P-labeled Meth3p primer was used in this analysis. The arrow indicates the position of the extension product, which corresponds to a region 441 to 454 nt upstream of the translation start site (see Fig. 2).