Fig. 4.

Blebbistatin inhibited LPS-induced activation of NMMHC IIA/Wnt5a/β-catenin signaling pathway in pulmonary endothelium in mice. Immunofluorescence staining for (A) NMMHC IIA, (B) Wnt5a, (C) β-catenin, (green) and CD31 (red) were performed on frozen lung sections, and the nuclei were stained with DAPI (blue). Scale bar =20 μm. The fluorescence intensity of these proteins in lung endothelium was quantified by Image J (n = 3). Data are shown as the means ± SD (A-C). n = 3. ^##P < 0.01 vs. the control group; **P < 0.01 vs. the LPS group.