Fig. 5.

Blebbistatin inhibited LPS-induced MLECs hyperpermeability. MLECs seeded on gelatincoated transwell inserts for 5–6 days formed a monolayer, which was administrated with blebbistatin (1 μmol/L) for 1 h prior to LPS (300 ng/ml) stimulation for 6 h. (A) The effect of blebbistatin on LPS-induced decrease in MLECs TEER was determined by Millicell-ERS resistance instrument. (B) The effect of blebbistatin on LPS-induced MLECs hyperpermeability was detected by EBA. (C–D) Effect of blebbistatin on LPS-induced decrease of VE-cadherin protein content in MLECs membrane was analyzed by flow cytometry. Data are shown as the means ± SD (A-B). n = 6. (C–D). n = 3. ^##P < 0.01 vs. the control group; **P < 0.01 vs. the LPS group.