Fig. 6.

Blebbistatin inhibited LPS-induced HUVECs hyperpermeability. HUVECs seeded on gelatincoated transwell inserts for 5–6 days formed a monolayer, which was administrated with blebbistatin (1 μmol/L)) for 1 h prior to LPS (5 μg/ml) stimulation for 6 h. (A) The effect of blebbistatin on LPS-induced decrease in HUVECs TEER was determined by Millicell-ERS resistance instrument. (B) The effect of blebbistatin on LPS-induced HUVECs hyperpermeability was detected by Evans blue-labeled albumin. (C) Effect of blebbistatin on LPS-induced decrease of VE-cadherin expression in HUVECs were measured by western blotting. Data are shown as the means ± SD (A-B). n = 6. (C). n = 3. ^##P < 0.01 vs. the control group; **P < 0.01 vs. the LPS group.