TABLE 1.
Plasmids used and constructed in this study
| Plasmid | Descriptiona | Source or reference |
|---|---|---|
| pCRII-TOPO | 3.9-kb cloning vector | Invitrogen |
| pCR-LUX | 409-bp luxS PCR product cloned into pCRII-TOPO | This study |
| pVA3000 | Suicide vector for Bacteroides; Emr | 25 |
| R751 | IncP plasmid used to mobilize vectors from E. coli to Bacteroides recipient; Tpr, Tra+ | 29 |
| pLR409 | pVA3000 containing an insertion of BamHI-XbaI fragment from pCR-LUX | This study |
a
Abbreviations: Emr, erythromycin resistant; Tpr, trimethoprim resistant; Tra+, self-transferable.