Table 1.
FRET substrate (μM) | Linear equations | Avg. velocity (ΔRFU/s) | ||
---|---|---|---|---|
35 | y=88.32x+5829.9 | y=93.12x+5766.6 | y=93.33x+5618.1 | 91.59 |
30 | y=85.91x+4913.4 | y=83.4x+5033.3 | y=81.4x+4999.8 | 83.57 |
25 | y=75.83x+4126.6 | y=78.59x+4019.9 | y=77.7x+4126.6 | 77.37 |
20 | y=69.21x+3699.2 | y=67.5x+3814.5 | y=67.8x+3644.9 | 68.17 |
15 | y=64.34x+3610.4 | y=63.21x+3673.2 | y=63x+3553.5 | 63.52 |
10 | y=49.26x+2882.2 | y=51.41x+2008.7 | y=51.53x+2698.7 | 50.73 |
5 | y=25.63x+1486.9 | y=27.81x+1402.8 | y=27x+1396.2 | 26.81 |
2.5 | y=10.66x+683.5 | y=10.5x+608.9 | y=10x+576.1 | 10.39 |
0 | 0 | 0 | 0 | 0 |