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. 2022 Oct 3;3(4):101794. doi: 10.1016/j.xpro.2022.101794

Table 1.

The initial velocity of Mpro enzyme reaction in the FRET assay

FRET substrate (μM) Linear equations Avg. velocity (ΔRFU/s)
35 y=88.32x+5829.9 y=93.12x+5766.6 y=93.33x+5618.1 91.59
30 y=85.91x+4913.4 y=83.4x+5033.3 y=81.4x+4999.8 83.57
25 y=75.83x+4126.6 y=78.59x+4019.9 y=77.7x+4126.6 77.37
20 y=69.21x+3699.2 y=67.5x+3814.5 y=67.8x+3644.9 68.17
15 y=64.34x+3610.4 y=63.21x+3673.2 y=63x+3553.5 63.52
10 y=49.26x+2882.2 y=51.41x+2008.7 y=51.53x+2698.7 50.73
5 y=25.63x+1486.9 y=27.81x+1402.8 y=27x+1396.2 26.81
2.5 y=10.66x+683.5 y=10.5x+608.9 y=10x+576.1 10.39
0 0 0 0 0