Fig. 5. Restoration of the Il1r1 gene in oligodendrocyte lineage cells does not lead to IL-1α-mediated neuroinflammation and oligodendrocyte loss.

a Quantification of restoration of Il1r1 gene expression in primary PDGFRα⁺ and O4⁺ oligodendrocyte lineage cells isolated by immunopanning from the uninjured spinal cord of adult Il1r1^r/r mice, who express an IL-1R1-knockout phenotype, and Pdgfra^CreER::Il1r1^r/r mice at 50 days post-tamoxifen treatment (n = 3 per group and each sample has a pool of 4 mice). b Quantification of the number of Ly6G⁺ neutrophils that infiltrated the spinal cord of WT, Il1r1^r/r and Pdgfra^CreER::Il1r1^r/r mice at 24 h post-injection of either PBS or rmIL-1α (n = 3–4 mice/group: n = 4 WT + PBS, n = 4 Il1r1^r/r + PBS, n = 4 Pdgfra^CreER::Il1r1^r/r + PBS, n = 3 WT + rmIL-1α, n = 4 Il1r1^r/r + rmIL-1α, n = 4 Pdgfra^CreER::Il1r1^r/r + rmIL-1α). c Quantification of the total number of Olig2⁺ CC1⁺ mature oligodendrocytes in the spinal cord white matter of WT, Il1r1^r/r and Pdgfra^CreER::Il1r1^r/r mice at 24 h post-injection of either PBS or rmIL-1α (n = 3–5 mice/group: n = 4 WT + PBS, n = 3 Il1r1^r/r + PBS, n = 3 Pdgfra^CreER::Il1r1^r/r + PBS, n = 3 WT + rmIL-1α, n = 3 Il1r1^r/r + rmIL-1α, n = 5 Pdgfra^CreER::Il1r1^r/r + rmIL-1α). Data are presented as mean values + /− SEM and statistical significance was determined by a two-way ANOVA followed by a Bonferroni post-hoc test (a–c). Pairwise comparisons and p-values are indicated in the graphs.