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. 2022 Sep 19;10:952670. doi: 10.3389/fbioe.2022.952670

FIGURE 1.

FIGURE 1

Mixed baculovirus carrying the miR-214 sponge sequence promotes osteogenic differentiation of ASCs (Li et al., 2017). (A,B) Schematic diagrams of four reporter gene plasmids were constructed to express Gaussia luciferase (Gluc) and firefly luciferase (Fluc). At the 3′-UTR site of Fluc, there were wild-type or mutant TAB2 (TAB2-wt or TAB2-mut) or CTNNB1 (CTNNB1-wt or CTNNB1-mut) sequences. (C) H&E staining was performed 5 weeks after femoral epiphyseal segment implantation in rats. (D) Alizarin red staining (ARS) and AdipoRed staining after virus transfection of adipose stem cells from ovariectomized rats (OVX-ASCs). (Mock, transduced without BV; LEBW, sustained BMP2 expression; 214S, co-transduction of OVX-ASCs with BacECre/Bac214S; Non-operated, rats without ovariectomy; Sham, ASCs were isolated from animals without OVX; H&E, hematoxylin and eosin.) Copyright 2017. Reproduced with permission from Springer Nature.