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. 2022 Sep 19;10:952670. doi: 10.3389/fbioe.2022.952670

FIGURE 4.

FIGURE 4

Silk fibroin/hydroxyapatite/graphene oxide–polyethyleneimine (SF/HAP/GO-PEI) scaffold carrying the miR-214 inhibitor promotes osteogenic differentiation (Ou et al., 2019). (A) Schematic diagram of the SF/HAP/GO-PEI scaffold carrying the miR-214 inhibitor to promote bone regeneration of bone defects. (B) SEM images of silk fibroin (SF), hybrid hydroxyapatite and SF scaffolds (SF/HAP), and SF/HAP/GO-PEI-miR-inhibitor (GPM). (C) CLSM fluorescence images showing non-GO-PEI-treated control and GO-PEI labeled with FITC (GO-PEI-FITC) in MC3T3-E1 cells. (D) Fluorescence images showing MC3T3-E1 cells treated with control, naked miR-inhibitor, Lipofectamine 2000, and GO-PEI after 24 h incubation. H&E staining (E) and MTC (F) were performed on the histological sections of the control group (defects without scaffolds served), SF, SF/HAP, and SF/HAP/GPM scaffolds after 4 and 12 weeks of implantation. Reproduced with permission from Ivyspring International Publisher.