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. 2022 Sep 19;13:1012548. doi: 10.3389/fgene.2022.1012548

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Copyright © 2022 Bao, Zhu, Fan, Ye, Li and Chai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qRT-PCR and Western blot analysis of SPAG6 and NM23 in osteosarcoma and adjacent normal muscle tissues (control). (A) SPAG6 and NM23 mRNAs were detected by qRT-PCR. (B) SPAG6 and NM23 proteins were detected by Western blotting in osteosarcoma and adjacent normal tissues. (C) Quantitative analysis was conducted, and the results were normalized against the levels of α-tubulin (DM1A). The data were presented with ±s (n = 6). OS: osteosarcoma; Ctr: control (adjacent normal muscle tissues). *p < 0.05 vs. control group.