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. 2022 Sep 19;13:1011965. doi: 10.3389/fgene.2022.1011965

FIGURE 1.

FIGURE 1

SNP rs58905141 and rs10499197 demonstrate allele-specific nuclear protein binding. EMSA using nuclear extracts from Jurkat, EBV B, and THP-1 cells stimulated with or without PMA/Ionomycin (P/I) for 2 h. Radiolabeled probes of (A,B) the non-risk (A) or risk (G) allele of rs58905141 or (C,D) the non-risk (T) or risk (G) allele of rs10499197 were incubated with nuclear extracts as indicated. (A,C) Images are representative of n > 8. (B,D) Densitometry was used to quantify nuclear factor-bound DNA probe; paired t-test: *p < 0.05, **p < 0.005; ***p < 0.001. (Figure Abbreviations: NE, nuclear extract; NR, non-risk allele; R, risk allele).