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. 2022 Sep 19;13:1011965. doi: 10.3389/fgene.2022.1011965

FIGURE 2.

FIGURE 2

SNP rs58905141 and rs10499197 reveal novel enhancer function. (A–F) Dual luciferase assay using Jurkat (A,D), EBV B (B,E), or THP-1 (C,F) cells transiently transfected with empty pGL4.23, pGL4.23 containing the ∼350 base pair enhancer with the non-risk (A) or risk (G) allele of rs58905141 (A–C) or the non-risk (T) or risk (G) allele of rs10499197 (D–F). (G–I) Dual luciferase assay using Jurkat (G), EBV B (H), or THP-1 (I) cells transiently transfected with empty pGL4.23 or pGL4.23 containing the 747 base pair enhancer with the combined non-risk (A,T) or risk alleles (G,G) of rs58905141 and rs10499197. In all experiments, cells were stimulated without or with PMA/Ionomycin for 2 h. Relative luciferase units were calculated as a normalized ratio of Firefly to Renilla; Student’s t-test, n > 3; *p < 0.05; **p < 0.01. (Figure Abbreviations: V, pGL4.23 vector-only; NR, pGL4.23 plus non-risk allele; R, pGL4.23 plus risk allele; P/I, PMA/Ionomycin).