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(A) The background fluorescence, (B) the signal-to-background ratio at 10 nM and (C) the fluorescence recovery rate at 10 nM of all fluorescent reporters. (D) Heat map comparison of endpoint fluorescence ratio with the different types of reporters of the Cas12a detection. Target DNA at 1 nM, 100 pM and 0, respectively for all cases (t = 4000 s).
