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. 2022 Sep 30;373:132746. doi: 10.1016/j.snb.2022.132746

Fig. 3.

Fig. 3

(A)The ORF1a gene and the N gene in the SARS-CoV-2 genome map used in this work. The real-time of fluorescence intensity and fluorescence value of ORF1a gene(B) and N gene fragment at 47 ℃ and 4000 s with using MB4 reporters. (C) The real-time of fluorescence intensity and Fluorescence value of ORF1a gene at 47 ℃ and 4000 s with the use of MB reporters with dual crRNAs when the ratio of Cas12a to crRNAs at 2. n = 3 replicates, bars represent mean ± S.D. *P < 0.05, * *P < 0.01, * ** *P < 0.0001, n.s. not significant.