Fig. 2.

Multiple sequence alignment (MSA) of SPAO with Savinase (WP_094423791.1), subtilisin Carlsberg (WP_020450819.1), and BPN' (WP_013351733.1). The alignment was calculated by clustal omega and drawn using ESPript 3.0 and Savinase (PDB: 1C9J) as a template. Solid green and blue bars indicate the signal peptide sequence and propeptide of SPAO. Secondary structure elements are presented on top (helices with squiggles, β‐strands with arrows, and turns with TT letters). Orange boxes show residues comprising the catalytic triad (Asp¹⁴⁵, His¹⁷⁵, Ser³²⁸; SPAO numbering).