Fig. 10.

Effect of NaCl on the activity of the purified SPAO, BPN', Savinase, and subtilisin Carlsberg. The activity was measured in standard buffer (pH 8.6) for suc‐AAPF‐pNA assay at 30 °C with different NaCl concentrations of 0–5 m. The maximum activity for each protease was considered as 100% activity; SPAO (closed circles; 1205 U·mg⁻¹), BPN' (squares; 560 U·mg⁻¹), Savinase (triangles; 757 U·mg⁻¹), and subtilisin Carlsberg (open circles; 846 U·mg⁻¹). The experiment was performed in triplicates, and data are given as mean values ± SD.