. 2022 Jul 6;12(10):1729–1746. doi: 10.1002/2211-5463.13457

Table 2.

Physicochemical parameters of the proteases.

Protease	Experimental pI	Arg	Asp	Glu	His	Lys	AB ratio ^a
SPAO	9.80	9	4	4	6	3	0.4
Savinase	9.80	8	5	5	7	5	0.5
subtilisin Carlsberg	8.00	3	9	5	5	10	0.8
BPN'	7.80 ^b	2	10	5	6	11	0.8

^{^a}

The AB ratio [AB = (Glu + Asp)/(Lys + His + Arg)] was calculated as described in R85].

^{^b}

pI of BPN’ according to Matsubara et al. [38].