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Purification analysis of the PorX proteins from F. johnsoniae and P. gingivalis. (a) Size-exclusion chromatography (Superdex 200 Increase) of PorXFJ (FJ) and PorXPG (PG). Both proteins elute at volumes appropriate for a monomer (∼60 kDa). Molecular markers, ferritin (440 kDa), conalbumin (75 kDa), carbonic anhydrase (29 kDa) and ribonuclease A (13.7 kDa), are indicated by a dashed gray line. (b) SDS–PAGE analysis demonstrates high purity levels of both protein homologs.
