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. Author manuscript; available in PMC: 2022 Oct 3.
Published in final edited form as: Cell Rep. 2022 Aug 23;40(8):111223. doi: 10.1016/j.celrep.2022.111223

Figure 4. Non-stem-like proliferating cells localize to the adenoma compartment.

Figure 4.

(A) UMAP plot of corticotroph cells showing cell-cycle gene scores per cell. We defined proliferating cells (Pros) for further study to have a cycle score >0.01.

(B) Dot plot showing 12 top expressed genes upregulated in Pros. These cells clearly expressed canonical corticotroph marker genes POMC and TBX19 in addition to cell-cycle genes.

(C) UMAP plot showing cell-cycle score across all six scRNA-seq patient samples, showing specificity to the C cluster and some Le cells, as well as localization to the NFPA, and PRL adenoma clusters.

(D) SOX2-positive cells are restricted to the cell cluster classified as FS cells and not expressed in the corticotroph compartment.

(E) Dot plot of select genes associated with pituitary stem cells (Cox et al., 2017; Fletcher et al., 2019) showing lack of expression in adenoma samples.