KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| EGFR | GeneTex Inc | Cat #GTX20231; RRID:AB_368210 |
| Phospho-EGFR Tyr992 | Cell signaling | Cat #2235s; RRID:AB_331708 |
| POMC | LifeSpan Biosciences | Cat #LS-C340619–100 |
| c-Myc | Santa Cruz | Cat #sc-40 AF546; RRID:AB_627268 |
| TBX19 | Thermo Fisher | Cat #MA5–31431; RRID:AB_2787067 |
| Noxa | Novus Biologicals | Cat #NB600–1159AF594; RRID:AB_10001419 |
| TSH | Abcam | Cat #ab64378; RRID:AB_1143452 |
| GH1 | Novus Biologicals | Cat #NBP2–53262 |
| LH | Novus Biologicals | Cat #NBP2–47733AF700 |
| Prolactin | Novus Biologicals | Cat #NBP3–08245AF488 |
| FSH | Novus Biologicals | Cat #NBP2–47734AF532 |
| DAPI | Thermo Fisher | Cat #D1306; RRID:AB_2629482 |
| CRHR1 | Invitrogen | Cat #720290 |
| EGFR A10 | Santa Cruz | Cat #sc-373746; RRID:AB_10920395 |
| Vinculin 7F9 | Santa Cruz | Cat #sc-73614; RRID:AB_1131294 |
| Erk 1/2 | Cell Signaling | Cat #4695; RRID:AB_390779 |
| Phospho Erk 1/2 Thr202/Tyr204 | Cell Signaling | Cat #4370; RRID:AB_2315112 |
| UCH-L1 | Santa Cruz | Cat #sc-271639; RRID:AB_10714950 |
| Noxa D8L7U | Cell Signaling | Cat #14766; RRID:AB_2798602 |
| Cleaved PARP | Cell Signaling | Cat #5625; RRID:AB_10699459 |
| Caspase 3 | Cell Signaling | Cat # 9662; RRID:AB_331439 |
|
Chemicals, peptides, and recombinant proteins | ||
| Clostridium histolyticum collagenase | Sigma-Aldrich | Cat #9001–12-1 |
| RPMI 1640 | Thermo Fisher | Cat #11875093 |
| FBS | Thermo Fisher | Cat #26140 |
| 1% penicillin-streptomycin | Gibco | Cat #15070063 |
| Human epidermal growth factor | Sigma-Aldrich | Cat #11376454001 |
| DMEM | Thermo Fisher | Cat #11965084 |
| Bortezomib | Millipore | Cat #CAS 179324–69-7 |
| Gefitinib | SelleckChem | Cat #ZD1839 |
| LDN-57444 | Sigma-Aldrich | Cat # 668467–91-2 |
| DMSO | Thermo Fisher | Cat #85190 |
|
Critical commercial assays | ||
| ACTH ELISA | MDBio products | Cat #M046006 |
| Prime PCR assay | BioRad | Cat #H384 |
| Cyclohexamide assay | Sigma-Aldrich | Cat #66–81-9 |
| CellTiterGlo | Promega | Cat #G9241 |
|
Deposited data | ||
| Bulk RNAseq and single-cell RNAseq data | GEO | GSE208112 |
|
Experimental models: Cell lines | ||
| Mouse: mCortEGFR: BALB/c | This paper | N/A |
| Mouse: mCortEV: BALB/c | This paper | N/A |
| Mouse: ATT-20 D16:6 | Dr. Steven Sabol, NHLBI | N/A |
|
Oligonucleotides | ||
| EGFR for 5’CCCATGCGGAACTTACAGGAA’3 | Sigma | Cat #VC00021 |
| EGFR rev 5’TTGGATCACATTTGGGGCAAC’3 | Sigma | Cat #VC00021 |
| POMC for 5’ATGCCGAGATTCTGCTACAGT’3 | Sigma | Cat #VC00021 |
| POMC rev 5’CCACACATCTATGGAGGTCTGAA’3 | Sigma | Cat #VC00021 |
| PMAIP1 for 5’GCAGAGCTACCACCTGAGTTC’3 | Sigma | Cat #VC00021 |
| PMAIP1 rev 5’CTTTTGCGACTTCCCAGGCA’3 | Sigma | Cat #VC00021 |
|
Other | ||
| Chromium instrument | 10× genetics | N/A |
| NextSeq sequencing machine | Illumina | N/A |
| TruSeq sequencing machine | Illumina | N/A |
| Infinium MethylationEPIC chip | Illumina | Cat #20042130 |