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. 2021 Oct 14;25(1):110–121. doi: 10.1007/s11307-021-01656-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2. — Tumor and normal tissue discrimination by signal intensity. a After image co-registration, pathologist-defined ROIs of normal tongue (NT), salivary glands (SG), and tumor were drawn on the gold standard H&E images. Signal intensity in the ROIs on corresponding untargeted fluorescence, targeted fluorescence, and BP images are compared for a representative tumor from each cell line. b The average signal from each ROI was plotted for all animals in three tumor groups. For clarity, only the statistical mean comparison with normal tissue is shown.