FIG. 1.

SDS-PAGE analysis of purified His10-GerBA protein. Synthesis of the His10-GerBA protein was induced in E. coli cells of strain BL21 λ(DE3) carrying plasmid pFE254, the soluble proteins were isolated, and GerBA was purified as described in Materials and Methods. An aliquot (8 μg) of the purified protein preparation (lane P) was run on an SDS-10% PAGE gel and visualized by Coomassie blue staining. The arrowhead points to the major species that was purified, and asterisks mark the smaller species, which are degradation products of the major protein (see text). Arrows indicate the migration positions of the molecular mass markers in kilodaltons (lane M).